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KMID : 0900919990230030239
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Korean journal of Animal Reproduction
1999 Volume.23 No. 3 p.239 ~ p.245
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Effects of Catalase and Cumulus Cells during In Vitro Maturation in Porcine Oocytes
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Lee S.-Y.
Sa S.-J.
Kim K.-J.
Cheong H.-T.
Yang B.-K.
Park C.-K.
Kim C.-I.
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Abstract
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The effect of catalase on in-vitro maturation in porcine oocytes with or without cumulus cells were studied. The maturation rates were not significantly different in medium with and without catalase during in vitro maturation of oocytes. However, the maturation rates of oocytes with cumulus cells were significantly higher (P＜0.05) than in oocytes without cumulus cells regardless of the presence of catalase. On the other hand, the maturation rate in oocytes cultured with cumulus cells for 48 h (57%) was significantly (P＜0.05) higher than in oocytes with cumulus cells for first 24 h period (42%) only. In another experiment, the maturation rate was significantly (P＜0.05) higher in medium containing catalase for last 24 h period only than in medium containing catalase for first 24 h period during in vitro maturation of ooytes with or without cumulus cells. But the oocytes matured to M-II stage were observed at 24 h of culture of oocytes without cumulus cells only. When oocytes with cumulus cells were cultured for 72 h, the maturation rates was significantly (P＜0.05) higher in medium with (79%) than without (65%) catalase during in vitro maturation. These results indicate that cumulus cells are necessary for in vitro maturation of porcine oocytes, catalase have effect according to the addition periods and can prevent aging of porcine oocytes during maturation in vitro.
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KEYWORD
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In vitro maturation, Catalase, Cumulus cells, Porcine
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